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15 mins 30 mins added advantage agar alcohol aldehyde ammonium acetate angles Araldite autoradiography carbon film caustic cells cent centrifuge Chapter chemical citrate coated colour containing cutting thin sections dense described desirable Diamond knives dilute distilled water double fixation drop electron microscope electron microscopy embed embedding medium emulsion enzyme ethanol evaporation exposure fixative floating floating film formaldehyde Formvar gelatin capsules glass knives glutaraldehyde grids knife light microscope liquid Luft material membranes methacrylate embedding method micrographs microscope slide microtome Millonig minutes molecules monomer mounted nucleic acids object obtained osmium tetroxide particles permanganate phosphatase phosphotungstic acid piece of tissue plastic platinum polymer polymerisation polymerisation damage post fixation potassium Potassium permanganate precipitate preparation problem procedure protein reaction refrigerator removed resins salts serial sections shadow small block sodium Solut1on solution specimen stabilise structure sucrose suitable techniques thick trimming uranyl acetate usually water bath water surface