ImmunophenotypingCarleton C. Stewart, Janet K. A. Nicholson Immunophenotyping Edited by Carleton C. Stewart and Janet K. A. Nicholson In the last twenty years there has been an explosion of immunophenotyping applications using flow cytometry in the clinical laboratory. Immunophenotyping offers essential information for clinicians and laboratorians about the uses of flow cytometers, identifying abnormalities in a variety of disorders, tools of immunophenotyping, assessing platelets in disease states, and much more. This second volume in the new series, Cytometric Cellular Analysis, comprises all cytometric methods currently used to study cellular function through reviews of the principles, theoretical background, and applications of these methods with particular reference to their use in clinical laboratories. Cellular analytical technologies have revolutionized our ability to identify, isolate, and functionally characterize single cells. This volume addresses one of the most important aspects of flow cytometry as it applies to the clinical setting. In addition, this comprehensive book: * Discusses methods used for quality controlling immunophenotyping trials * Reviews various approaches to the use of flow cytometers to quantify fluorescence * Explains how cell surface receptors differentiate between normal and abnormal tissues for diseases including lymphoma, leukemia, and AIDS * Describes a new, sensitive flow cytometric immunophenotyping assay for cross-matching in the case of transplants Imraunophenotyping conveys to researchers, lab supervisors, and scientists working in the areas of flow cytometry, cytology, pathology, hematotogy, immunology, and immunopathology why this outstanding technology is so vital to biomedical research. |
Contents
Kinetics of Antibody Binding to Cells | 1 |
An Uneasy Alliance | 23 |
Flow Cytometric Analyses of Cell Phenotypes | 49 |
Copyright | |
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Common terms and phrases
abnormal activation acute addition analysis antibody antigen antigen expression applications approach assay associated beads binding blasts blood bone marrow calibration cells cellular changes characterization Clin clinical combination compared concentration correlation cytoplasmic defined demonstrated detection determine diagnosis differentiation disease epitope et al evaluation example expression flow cytometry fluorescence fluorochrome function gating Group human identified Immunol immunophenotyping important increase individual infection intensity labeled laboratories leukemia leukemic leukocytes levels light lineage lymphoblastic lymphocytes lymphoma markers mature measured membrane method molecules morphologic myeloid negative normal parameters patients patterns performed peripheral phenotype platelet population positive present probe problem protein quantitative reagents receptor relative reported residual response samples scatter sensitivity shown signal significant specific specimens stage staining standards studies surface T-cell techniques tion transplantation variability