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acetone acetone wash acid-sodium borate buffer activated charcoal Active fractions aglycone air stream amyl alcohol anthrone aqueous Ascochitine Ascochyta chrysanthemi toxin Ascofuranone Ascotoxin Bio-Gel P-2 bioassayed boric acid-sodium borate butanol carbonum cellulose chromatograms chrysanthemum coincident with toxin column of Bio-Gel compounds containing AC toxin culture filtrate DEAE-cellulose Deproteinated Toxin Desorbed Toxin eluant eluted with glass enzyme ethanol Figure filter fluorescence fractions were pooled free reducing Fusicoccin gave symptom ratings glass distilled water glycoside inactivated l-ml leaf spots ligulicola line of origin low molecular weight Mallinckrodt Chemical mannose mg/ml ml aliquots ml of glass molecular sieve molecular sieve gel NaBH NaCl ninhydrin organic liquids oxalic acid paper chromatography pathogen peak Phytopathology phytotoxins produced Purified AC Toxin Purified Toxin reaction mixture reactive material reagents residue rotary evaporator sample Sigma Chemical soluble solvent systems sorbed supernatant taken to dryness tetranitromethane toxic toxin activity toxin preparations trifluoroacetic acid veinal necrosis victorin Whatman