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ablation AChE Acta Embryol adhesive papillae amphibian ampullae animals anti-ES anti-ES-binding surface Ascidia malaca ascidian Batch Biol blastomeres blastula buds Bufo Ca-free caudal cephalothoracic segments chick embryo crassispina culture cytoplasmic Department of Biology developmental stages differentiation Discoglossus pictus ecdysone ecdysteroids eggs embryonic development embryos endodermal enzyme ependymal explants extracts fertilization envelope formation fractions ganglion germ disc glands grafts gregaria growth hormone horseshoe crab hyaline layer incubation induced inhibition inhibitors isolated Italy larvae LDH activity lectin lymphocytes melatonin membrane mesodermal mesonephros metamorphosis microtubules mitochondria monsters mRNA nerve fibers neurons normal observed obtained oozooids operation operculum Ortolani Palermo photoperiod pictus tadpoles pineal present prolactin protein Rana reaction regeneration regenerative renin renin activity sea urchin seawater short photoperiod skin species spinal cord stained synthesis tadpoles tail resorption tissue trunk tubulin tunic University vitro Xenopus laevis yolk zooids Zool