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activity added amino acids amount of biotin animal aoid aotive arginine aspartio acid assay for biotin bacteria Biol BIOLOGICAL CHEMISTRY Vol biotin content biotin deficiency biotin-low ration casein Casitone cent Chem chemically defined medium Clostridium compound containing culture curve cystine Difco diluted egg white eluate factor Elvehjem ethyl linolate experiments feces fibrin Fildes filtered filtrate free acid G. M. SHULL glucose high concentrations Hutchings hydrolyzed hydrolyzed casein inactivation of biotin incubation inoculum Journal of Biological Lactobacillus casei linoleio method micromi mixture norit nutrition obtained oleic acid oleio oxalaoetate oxidation oxybiotin pantothenic pantothenic acid partial protein digests peroxide-treated biotin phenylalanine prepared present purified ration quantities rancid rancid fat rats replacing biotin response riboflavin room temperature sample Snell sporogenes vitamin stimulatory effect stimulatory factor sulfaguanidine synthesis of biotin thioglyoolate tryptophan tube turbidities Tween tyrosine University of Wisconsin vitamin W. H. PETERSON whioh yeast extract