Procedures in Nucleic Acid Research, Volume 1Guilio Leonard Cantoni, David R. Davies Harper & Row, 1966 - Nucleic acids |
Contents
Deoxyribonucleases Endonucleases | 3 |
Ribonuclease from Streptomyces erythreus | 14 |
JOHN ABELSON Department of Biophysics The Johns Hopkins University | 19 |
Copyright | |
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2-mercaptoethanol absorbance acetate buffer acetone acid deoxyribonuclease Acta added addition amino acid Ammonium Sulfate Fractionation amount approximately assay Biochem Biol Biophys cells Chem chromatography collected by centrifugation column concentration containing crude extract DEAE DEAE-cellulose deoxyribonuclease dialysis dialyzed diluted dissolved DNA polymerase EDTA eluate eluted enzymatic enzyme activity enzyme solution equilibrated Escherichia coli ethanol exonuclease filter gradient homogeneous hydrolyzed hydroxylapatite incubation liters medium method methyl mg/ml Mg2+ MgCl2 ml/hr molarity molecular weight NaCl nuclease nucleic acid nucleotide obtained oligonucleotides peak perchloric acid phenol phosphatase phosphodiesterase phosphomonoesterase poly potassium phosphate potassium phosphate buffer precipitate preparation Proc protein radioactivity reaction mixture reagent removed by centrifugation residues rev/min ribonuclease ribonuclease activity ribosomes RNA Synthetase saturation Sephadex sodium specific activity sRNA Step stirring streptomycin substrate sucrose supernatant fluid supernatant solution Table temperature tion transferase Tris buffer Tris-HCl buffer tRNA tubes venom washed