Nonmammalian Genomic Analysis: A Practical Guide (Google eBook)

Front Cover
Bruce Birren, Eric Lai
Academic Press, Sep 25, 1996 - Science - 353 pages
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Offering detailed protocols for those needing to construct a variety of maps and isolate genes, this unique book is intended to popularize the new techniques of genome analysis derived from the Human Genome Project. The power of these new methods is often most striking when applied to problems outside of human genetics, particularly the nonmammalian systems on which many researchers focus. Many of these organisms are economically important and biologically rich.
Nonmammalian Genomic Analysis: A Practical Guide covers the "how to" aspects of preparation, handling, cloning, and analysis of large DNA and the creation of chromosome and genome maps. This lab manual facilitates the transfer of these technologies to small "low tech" environments and allows them to be used by those with no background in genome mapping or large-fragment cloning. Like having a local expert, this collection provides procedures for anyone, anywhere, and allows the replication of others' success.

Key Features
* Includes detailed and clearly-written step-by-step protocols
* Evinces expected results and offers trouble shooting advice
* Provides techniques appropriate for small laboratories as well as those with limited resources
* Covers a broad variety of cloning systems, including single copy vectors
* Discusses a diverse range of organisms, from prokaryotes to eukaryotes, from single-celled organisms to highly complex organisms
  

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Contents

Chapter 1 Introduction to PulsedField Gels and Preparation and Analysis of Large DNA
1
Chapter 2 Electrophoretic Karyotyping in Fungi
25
Chapter 3 Isolation and Analysis of HighMolecularWeight DNA from Plants
61
Chapter 4 Generating and Using DNA Markers in Plants
75
Chapter 5 Genome Mapping of Protozoan Parasites by Linking Clones
135
Chapter 6 Macrorestriction Mapping and Analysis of Bacterial Genomes
165
Chapter 7 Cosmid Cloning with Small Genomes
197
Chapter 8 Construction of PI Artificial Chromosome PAC Libraries from Lower Vertebrates
223
Chapter 9 The Selection of ChromosomeSpecific DNA Clones from African Trypanosome Genomic Libraries
257
Chapter 10 Analysis of the Dictyostelium discoideum Genome
293
Chapter 11 Integrated Genome Mapping by Hybridization Techniques
319
Index
347
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