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Culture of Animal Cells:

A Manual of Basic Technique and Specialized Applications (Google eBook)
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John Wiley & Sons, Feb 2, 2011 - Science - 1170 pages

This is the sixth edition of the leading text in the basic methodology of cell culture, worldwide. Rigorously revised, it features updates on specialized techniques in stem cell research and tissue engineering; updates on molecular hybridization, somatic cell fusion, hybridomas, and DNA transfer; new sections on vitrification and Organotypic Culture, and new chapters on epithelial, mesenchymal, neurectodermal, and hematopoietic cells; germs cells/stemcells/amniocytes; and non-mammalian/avian cells. It is written for graduate students, research and clinical scientists, and technicians and laboratory managers in cell and molecular biology labs and genetics labs.

PowerPoint slides of the figures as well as other supplementary materials are available at a companion website: www.wiley.com/go/freshney/cellculture

  

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Contents

1 Introduction
1
2 Biology of Cultured Cells
11
3 Laboratory Design Layout and Equipment
25
4 Equipment and Materials
37
5 Aseptic Technique
57
6 Safety Bioethics and Validation
71
7 Culture Vessels and Substrates
89
8 Defined Media and Supplements
99
20 Quantitation
335
21 Cytotoxicity
365
22 Specialized Cells
383
23 Stem Cells Germ Cells and Amniocytes
433
24 Culture of Tumor Cells
463
25 ThreeDimensional Culture
481
26 Scaleup and Automation
497
27 Specialized Techniques
517

9 SerumFree Media
115
10 Preparation and Sterilization
133
11 Primary Culture
163
12 Subculture and Cell Lines
187
13 Cloning and Selection
207
14 Cell Separation
227
15 Characterization
239
16 Differentiation
269
17 Transformation and Immortalization
279
18 Contamination
299
19 Cryopreservation
317
28 Training Programs
533
29 Problem Solving
569
30 In Conclusion
593
Appendix I Calculations and Preparation of Reagents
595
Appendix II Sources of Equipment and Materials
603
Appendix III Suppliers and Other Resources
623
Appendix IV Glossary
633
Appendix V Crosscontaminated or Misidentified Cell Lines
639
Appendix VI General Textbooks and Relevant Journals
661
References
663
Copyright

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About the author (2011)

R. IAN FRESHNEY, PhD, is an honorary Senior Research Fellow in the Centre for Oncology and Applied Pharmacology, part of the Cancer Research UK Beatson Laboratories at the University of Glasgow. He is the author or editor of numerous books and a world-renowned expert on cell culture technique.

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