Characterization of the Intron-encoded Endonuclease I-PpoI from Physarum PolycephalumCornell University, 1994 - 236 pages |
Contents
Recognition of the IPpol Homing Site | 34 |
Physical Studies of the IPpoIDNA Complex | 56 |
The nature of IPpol and Pp LSU 3 in vivo | 79 |
Copyright | |
Common terms and phrases
absence activity adenine amino acid amount analysis appears approximately assays bacteriophage band bases binding blot bound cleavage cleavage site cleaved close complex conserved contacts containing contrast cytoplasmic deletion described detected determined DNA sequences EcoRI EcoRV EDTA electrophoresis encoded ends exon experiments expression extracts footprinting fraction fragment gene group I intron helix homing endonucleases I-PpoI ORF I-Ppol I-Scel identical identified immunoblotting included incubated indicated insertion point interaction interference intron homing intron insertion intron-encoded known ladder lanes major methylation minor groove mobility molecule MPEā«Fe(II mRNA mutants nature nuclear nucleotides observation Physarum plasmid position presence probe processing protection protein provides purified rDNA reactions recognition sequence recognize region represents restriction enzymes result ribosomal ribozyme self-splicing similar specific splicing strain strand structure studies suggests symmetry target translation vitro vivo yeast