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30 minutes acute adenoviruses agent animal antibiotics antibody antigen antigen dilution antigen titration antiserum appendix approximately blood bottles buffered cell suspension CF test Chick embryo common cold complement controls COMPLEMENT FIXATION containing described disc embryo encephalitis enteroviruses erythrocytes fever FIGURE flask fluorescent antibody forceps growth medium Hanks BSS hemadsorption HEMAGGLUTINATION hemolysin hemolysin dilutions host cell identification Immediately after onset inclusion conjunctivitis incubator indicated infection influenza influenza viruses inoculation isolation kidney material membrane method monkey kidney cells monolayer mouse neutralization test obtained onset 2-4 wk parainfluenza patients pipet Place poliovirus prepared procedures psittacosis reagents refrigerator Remove respiratory disease rickettsiae room temperature routine saline satisfactory sensitized cells serologic Serologic test serum sheep cells solution specific specimens sterile stool strains suckling mice susceptible swab Throat washing tissue culture titer titration trypsin usually viral virus dilution virus suspension viruses volume yolk sac