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Howard Weetall 67 Fermentative Production of InjectableGrade Calcium Gluconate
activity adsorbed adsorption afﬁnity American Chemical Society amino acids antibody antigen aqueous assay batch Bioeng bioreactor Biotechnol bubble bubble film buffer butanol cell concentration cell cycle cell density cell growth cell lines cellulase Chem Chemical Engineers coefficient column constant copolymer cycle decrease dextran diffusion EDTA effect elution enzyme equation ethanol experimental experiments extraction feed fermentation ﬁrst ﬂasks ﬂow ﬂuid ﬂuorescence fraction gel beads glucose glutamine growth rate hepatocytes hybridoma hydrophobic immobilized cell increased incubation initial interactions kinetics layer liquid mass transfer measured medium membrane metabolic method moisture content molecular weight naphthalene observed obtained oxygen parameters particles perfusate phase phosphate plasmid polymer predicted pressure production protein reaction reactor recombinant sample secretion rate serum shown in Figure signiﬁcant solubility solution solvent speciﬁc starch substrate surface suspension synthesis Table temperature values variables virus volume yeast