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100 ml volumetric amin analytical grade Ascorbic acid assay Autosampler e.g. WATERS Biotin bring to volume buffer solution Calcium pantothenate Carotenoids Compact de Luxe Concentration of standard Cyanocobalamin dard detector e.g. LINEAR Dry Vitamin Premixes filter flask accurately weigh Folic Acid g/kg HPLC HPLC Modules Instruments and Conditions IU vitamin kHz e.g. Compact Luxe TUC ISO Millipore Corp minutes ml centrifuge tubes ml of methanol ml volumetric flask Mobile phase Mono or double n-hexane Niacin Niacinamide Peak area Phytomenadione pipette possible all reagents previtamin D3 pulse-free pump e.g. pump e.g. Mod Pyridoxine reagents of analytical Results CV Riboflavin sample solution solution in mcg/ml Spectra Physics standard in mg standard solution stock solution Ultrasonic water-bath USP reference standard Variation of Results vitamin A acetate vitamin A palmitate Vitamin B2 vitamin D3 volume with ethanol volume with water volumetric flask accurately WATERS WISP 712 wavelength Weight of sample