Gene Cloning: An Introduction
An updated introduction to gene cloning and recombinant DNA technology for undergraduates, with an illustrated explanation of all the important concepts, avoiding jargon. It covers techniques for studying cloned genes, in particular with a description of methods for analyzing gene regulation.
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Why gene cloning is important
plasmids and bacteriophages
Purification of DNA from living cells
11 other sections not shown
adaptor amino acid ampicillin antibiotic autoradiograph bacterial cell bacteriophage bacterium base pairing biotechnology carried cDNA cell extract centrifugation chromosomal DNA cleave cloned gene cloning vectors coded codons colonies complementary contain control sequence copy number cosmid culture cystic fibrosis cystic fibrosis gene defective deletion density digestion DNA fragment DNA sequencing EcoRI enzyme example expression vector foreign gene gel electrophoresis gene cloning gene cloning experiment genome genomic library host cell hybridization probing identified infection cycle insertional inactivation insulin introns labelled lacZ ligase ligation medium membrane method molecular molecule Figure mRNA mutation normal nuclease nucleic acid nucleotide nucleotide sequence obtained oligonucleotide packaging phage phage DNA phage particles plaques plasmid plasmid DNA polylinker polynucleotide produced promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication restriction endonucleases restriction sites result RFLP selectable marker specific sticky ends strand structure synthesis techniques transcription transformed translation product types virus viruses vitro yeast