Gene Cloning : An IntroductionAn introductory textbook updated to incorporate advances made since the first edition was published in 1986, but retaining its mission to serve undergraduates with no previous experience of the subject and experienced researchers new to gene cloning. Annotation copyrighted by Book News, Inc., Portland, OR |
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Page 97
... plaques on a lawn of bacteria ( Figure 5.11a ) . Each plaque is a zone of clearing produced as the phage lyse the cells and move on to infect and eventually lyse the neighbouring bacteria ( Figure 5.11b ) . Both A and M13 form plaques ...
... plaques on a lawn of bacteria ( Figure 5.11a ) . Each plaque is a zone of clearing produced as the phage lyse the cells and move on to infect and eventually lyse the neighbouring bacteria ( Figure 5.11b ) . Both A and M13 form plaques ...
Page 98
... plaques ( c ) M13 plaques Lawn of confluent bacterial growth A plaque - a zone of clearing Lawn of bacteria Plaque - all the bacteria are lysed , leading to a high titre of bacteriophages Plaques contain slow - growing bacteria + M13 ...
... plaques ( c ) M13 plaques Lawn of confluent bacterial growth A plaque - a zone of clearing Lawn of bacteria Plaque - all the bacteria are lysed , leading to a high titre of bacteriophages Plaques contain slow - growing bacteria + M13 ...
Page 120
... plaques Plaque formed by mutant phage Only 3 EcoRI sites Repeat infection with mutant phage No EcoRI sites [ 8 ] Few more plaques Second mutant phage strain Figure 6.14 Using natural selection to isolate à phage lacking EcoRI ...
... plaques Plaque formed by mutant phage Only 3 EcoRI sites Repeat infection with mutant phage No EcoRI sites [ 8 ] Few more plaques Second mutant phage strain Figure 6.14 Using natural selection to isolate à phage lacking EcoRI ...
Contents
Why gene cloning is important | 3 |
plasmids and bacteriophages | 12 |
Purification of DNA from living cells | 27 |
Copyright | |
12 other sections not shown
Common terms and phrases
amino acid antibody autoradiograph bacteriophage bacterium BamHI base pairing biotechnology carried cDNA chromosomal DNA cloned gene cloning vectors coded codons coli colonies complementary containing control sequence cosmid culture cystic fibrosis cystic fibrosis gene defective deletion digestion DNA fragment DNA sequencing double-stranded EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression gene Figure genomic library higher organisms host cell hybridization probing identified infection insulin introns labelled lacZ LEU2 ligated M13 vector medium membrane method molecular molecule Figure mRNA mutagenesis mutation normal nuclease nucleotide nucleotide sequence obtained oligonucleotide phage particles plant cells plaques plasmid polylinker polymerase polynucleotide produced promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication restriction endonuclease restriction fragment restriction sites result RFLP selectable marker signals single-stranded DNA specific sticky ends T-DNA techniques Ti plasmid transcription transformed translation product trpA types unique restriction vaccine virus viruses vitro vitro mutagenesis yeast