Basic Methods in Molecular BiologyThe new edition of this popular book emphasizes the decisions that need to be made to select one procedure over another. |
Contents
பயி | 11 |
Cloning Vectors and Bacterial Cells | 23 |
Section 51 Rapid DNA Preparation | 42 |
Copyright | |
23 other sections not shown
Other editions - View all
Basic Methods in Molecular Biology Leonard G. Davis,Mark D. Dibner,James F. Battey Snippet view - 1986 |
Common terms and phrases
acid acrylamide agar agarose gel agarose gel electrophoresis aliquot ampicillin apparatus autoclaved autoradiography bacterial bacteriophage BamHI band cDNA cells centrifugation chloroform cloning vector coli column concentration containing CsCl described in Section DESCRIPTION This method digestion dilution Discard supernatant DNA fragments DNA polymerase DNA sample EcoRI EDTA electroelution enzyme ethanol ethidium bromide eukaryotic extraction gene genomic DNA glass H₂O hybridization Incubate insert LB medium ligase ligation lysate METHODS In Advance mg/ml MgSO4 minigel molecular mRNA NaCl NC filter nick translation nucleotide overnight phage pipette plaques plasmid plasmid DNA plastic plate polylinker precipitate procedure protein purified radioactive reaction REAGENTS Remove upper aqueous RNase room temperature screening Section 5-4 sodium acetate solution Southern blot SPECIAL EQUIPMENT specific Spin in microcentrifuge SS-phenol SS-phenol/chloroform step sterile Store at 20°C subcloning supernatant tissue Transfer tRNA upper aqueous phase vacuum volume wash