Synthesis of Electron Dense Labels and Structural Analysis of Multiprotein Aggregates |
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Page 83
... Radioactivity Measurement Radioactivity of a [ 14C ] -labeled aqueous samples was measured by adding the sample in a total volume of 1 mL of H20 to a glass scintillation vial containing 15 mL of Aquasol scintillation fluid and counting ...
... Radioactivity Measurement Radioactivity of a [ 14C ] -labeled aqueous samples was measured by adding the sample in a total volume of 1 mL of H20 to a glass scintillation vial containing 15 mL of Aquasol scintillation fluid and counting ...
Page 97
... radioactivity showed that 25 % of the gold clusters appeared in the flow through and contained no radioactivity . This was presumably unreacted icosa ( N - acetyl ) -1 . The remaining 75 % of the material was eluted in fractions 19 - 24 ...
... radioactivity showed that 25 % of the gold clusters appeared in the flow through and contained no radioactivity . This was presumably unreacted icosa ( N - acetyl ) -1 . The remaining 75 % of the material was eluted in fractions 19 - 24 ...
Page 112
... radioactivity of the complex . shown in Table IV , no such decrease occurred during 40 hr . Conversion of Icosa ( N ... radioactivity was released from the complex upon further reaction with hydroxylamine . This meant that Table IV ...
... radioactivity of the complex . shown in Table IV , no such decrease occurred during 40 hr . Conversion of Icosa ( N ... radioactivity was released from the complex upon further reaction with hydroxylamine . This meant that Table IV ...
Contents
Complexes by Electron Microscopy | 15 |
Materials and Methods | 66 |
66 | 75 |
13 other sections not shown
Common terms and phrases
acetic anhydride acylation alkylating amino groups avidin avidin molecules binding sites Bio-Gel P-6 Bleile bovine heart buffer CaJacob Chapter chemical chromatography citraconic anhydride cm column Coli PDH complex column of Sephadex component enzymes cross-linked derivatives desalted described dihydrolipoyl transacetylase dimeric E₁ E1 and E3 E₁ or E3 E₂ E2 core E2 enzymes E2E3 subcomplex E3 subunits EDTA electron dose electron-dense labels equilibrated and eluted fractions Frey gel electrophoresis glutaraldehyde gold clusters gold complex henicosa Histogram icosa N-acetyl)-1 images ligands lipoyl mass of PDH mixture molecular weight mono monomeric particle mass measurements PMSF point group prepared presence primary amino groups procedure protein purified Pyruvate Dehydrogenase Complex radial mass analysis radioactivity ratios reaction Reardon Reed rotary evaporation sample scanning transmission electron scattered electrons SDS-PAGE SDS-polyacrylamide gel electrophoresis shell masses solution SP-Sephadex C-25 species specimen STEM structural subunit binding domain symmetry synthesis transmission electron microscopy undecagold clusters