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Self Assembly In Vitro of Purified CANC Proteins from Rous Sarcoma
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aggregates AMBD APR amino acid ASLV assembled in vitro assembled particles assembly domains assembly pathways avian budding virion buffer capsid cellular factors centrifugation core crosslinking cylinders cylindrical particles cytoplasm D37I and 3H data not shown dialysis dimer electron microscopy Es-Bg Figure fragments Gag D37N Gag molecules Gag polyprotein Gag proteins genomic RNA helical helix HIV CA-NC HIV-1 Gag human immunodeficiency virus immunodeficiency virus type interactions internal deletion lane M-PMV mature membrane binding domain morphology mutants mutations NaCl NC domain negative staining nucleocapsid nucleocapsid protein observed particles assembled pellet perinuclear location pET3xc plasma membrane precipitate protease provirus purified R-3J PR region release retroviruses ribonucleoprotein RNA binding RNA strand RNase Rous sarcoma virus RSV and HIV-1 RSV CA-NC RSV Gag sequence spherical particles structure suggest thin sectioning transfected uranyl acetate viral proteins virion Virol Virology virus-like particles viruses vitro assembly system vivo Xh-No APR