DNA Transfer to Cultured Cells

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Katya Ravid, R. Ian Freshney
John Wiley & Sons, Aug 14, 1998 - Science - 296 pages
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DNA transfer to cultured cells

Edited by Katya Ravid and R. Ian Freshney

Rapid advances in DNA transfer technology have transformed many disciplines, ranging from molecular genetics to biotechnology. Scientists now have the means to introduce copies of genes into different cell types, then detect the expression of these genes in the cell. It is now possible to regulate cell growth that may lead to cancer, develop new biopharmaceuticals, and apply knowledge about the role of genes in cell processes to basic research in molecular genetics.

DNA Transfer to Cultured Cells is the first quick reference to all of the established techniques for the transfer of genetic material to cells in vitro. Featuring contributions by leading researchers in the field, this detailed guide walks the reader through a variety of DNA transfer methods, describes their application to specific cell types, and integrates aspects of molecular biology with tissue culture. Offering overviews and detailed protocols for the techniques under discussion in each of its sections, this book covers an exceptionally broad array of topics, including:
* Viral infection
* Electroporation
* Phosphate precipitation
* DEAE Dextran
* Liposomes
* Yeast artificial chromosomes (YACs)
* Whole chromosome transfer
* Enhanced expression.

Special sections at the end of each chapter list suppliers for necessary reagents and materials. This easy-to-use, self-contained guide addresses key developments of recent years as well as emerging trends in DNA transfer. For practical applications in cell biology, genetics, heredity, biotechnology, or evolution, DNA Transfer to Cultured Cells is a unique and unparalleled resource.
  

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Contents

Baculovirus Expression and the Study of
27
Electroporation of DNA into Cultured Cell Lines
55
DNA Transfer to BlastulaDerived Cultures
69
Transfection of Rat Adipose Cells
93
Calcium Phosphate Transfection
111
Optimization of Calcium Phosphate
125
Strontium Phosphate Transfection
147
DEAEDextran Transfection
179
Cationic Liposomes
194
Discussion
208
Transfection of Mammalian Cells with Yeast
213
Mapping Human Senescence Genes Using
237
Methotrexate and DHFR Amplification
265
List of Suppliers
283
Copyright

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Page 261 - In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain.
Page 279 - RT (1978). Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells.

About the author (1998)

GORDANA VUNJAK-NOVAKOVIC, PhD, is Professor in the Department of Biomedical Engineering at Columbia University, New York. She is the author of more than 125 papers and twenty-five book chapters on biotechnology, biomechanics, orthopedics, and tissue engineering.

R. IAN FRESHNEY, PhD, is Senior Research Fellow in the Centre for Oncology and Applied Pharmacolgy at the University of Glasgow. He is the author or editor of numerous books and a world-renowned expert on cell culture technique.