Gene Cloning: The Mechanics of DNA ManipulationThis book was originallyconceived in the form ofa second edition ofa volume published in 1980 in Chapman and Hall's 'OutllneStudies in Biology' series and entitled Genetic Engineering - Cloning DNA.It very rapidly became apparent that with the impact ofrecombinant DNA techniques being feIt in so many areas ofblology, it was going to be difficultifnotimpossible to keepthe bookwithin the space confines of these little monographs. The stays were therefore loosened and the book expanded comfortably to its present size.I hope that this extra space has allowed me to clarify sections ofthe text that were 'heavy going' in the earlierversion.Theextraspace has certainlyallowed me to cover topics that were not mentioned at all in the earlier book. These are primarily to be found in Chapters 7 and 8, which cover the rapid advances that have been recently made in the use ofplantand animal cells as hosts for recombinant DNAmolecules. The develop- ment ofother vectors has certainly not stood still over the past four years.This has necessitated a thorough revision ofChapters 3 and 4, which deal with bacteriophage and bacterial plasmid vectors. Numerous techniques for in vitromutagenesis have now been tried and tested allowing me to givecomprehensive coverage ofthisarea in Chapter 2 along with the biochemical techniques used to construct recombinant DNA molecules. Readers with some background knowledge of the approaches to gene cloning will be able to go straight toapart ofthe book in whichthey are specificallyinterested. |
Contents
Recombination and mutagenesis of DNA in vitro | 21 |
Bacteriophage vectors | 51 |
References | 81 |
Copyright | |
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acid annealed antigen bacterial bacteriophage BamHI base pair carrying cDNA cDNA cloning chromosomal chromosomal DNA cleaved cloned DNA cloning vectors codon ColEl constructed containing deletion detected developed digestion DNA ligase DNA molecules DNA polymerase DNA segments DNA sequences Drosophila EcoRI efficient encoded enzyme Escherichia coli eukaryotic eukaryotic genes expression foreign DNA functional gene product genetic genome HindIII host hybrid infection insertion intervening sequences introduced isolated lacZ gene ligation lysogens mammalian mapping marker molecular mouse cells mRNA mutagenesis mutations Natn Acad nucleotide octopine oligonucleotide operon peptide phage plaques plasmid DNA plasmid vectors probe Proc prokaryotic promoter protein PstI reading frame recombinant DNA region replication resistance restriction endonuclease restriction fragment S1 nuclease selected splicing ẞ-galactosidase ẞ-globin gene ß-lactamase strain strand synthesis T-DNA techniques terminal Ti plasmid transcription transformation transposon tumour vaccinia viral virus vitro wild type yeast