Gene Therapy Protocols: Volume 1: Production and In Vivo Applications of Gene Transfer Vectors, Volume 1; Volume 433
Humana Press, Apr 24, 2008 - Medical - 386 pages
In this book internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors and Design and Characterization of Gene Transfer Vectors. Readers will find a comprehensive resource of current and emerging methods for the production of viral and non-viral gene transfer vectors, as well as detailed protocols for applications in stem cell biology, cancer research and infectious disease.
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Production of Retroviral Vectors for Clinical Use
Oncolytic Adenoviruses for Cancer Gene Therapy
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adeno-associated virus adenovirus aliquot animal antibody assay blotting bone marrow cancer cassette cell culture cell line centrifuge chitosan concentration containing copolymers density dilution dishes DMEM dystrophin electroporation filter flask flow cytometry fluorescent gene delivery gene expression Gene Ther gene therapy gene transfer genetic genome harvest HDAd hematopoietic human imaging Incubate injection Invitrogen lentiviral lentiviral vectors lipid liposome luciferase medium membrane Methods mg/ml mice Molecular monolayer mouse muscle nanoparticles Note optimal packaging cell pellet pipette plaques plasmid plasmid DNA plate polyplex preparation producer cells production protein protocol pseudotyped Qiagen reagent recombinant replication reporter gene Resuspend retroviral vector room temperature sample sequences shRNA siRNA solution stem cells sterile Subheading supernatant syringe therapeutic tissue culture titer transcription transduction transduction efficiency transfection Transfer Vectors transgene tube tumor ug/ml vial viral DNA viral vectors virus vitro vivo volume VSV-G