Sampling. Project design. Collection and storage of tissues. Molecular techniques. Proteins: isozyme electrophoresis. Chromosomes: molecular cytogenetics. Nucleic acids I: DNA-DNA hybridization. Nucleic acids II: the polymerase chain reaction. Nucleic acids III: analysis of fragments and restriction sites. Nucleic acids IV: sequencing and cloning. Analysis. Intraspecific differentiation. Phylogenetic inference. Applications of molecular systematics: the state of the field and a look to the future.
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0.2 M Tris-HCl acetate agar overlay agarose allele frequencies allozyme amino acid amplification analysis Avise bands branch bridization buffer cells Centrifuge Chapter characters chromosomes cloning collections coverslip cpDNA dehydrogenase denaturation detect digestion Dimer distance divergence DNA hybridization DNA sequences EDTA electrophoresis enzyme estimate ethanol evolution evolutionary example Figure filter fragments freezer g/liter gel slice gene genetic genome genotypic Harris and Hopkinson heterozygotes Hillis homologous homoplasy Hopkinson 1976 hybridization Incubate inferred intron isozymes labeled likelihood liquid nitrogen loci locus markers matrix methods mg/ml NBT mg/ml PMS microsatellite molecular systematics mtDNA nick translation node nuclear nucleotide optimal parsimony patterns phylogenetic phylogeny plastic polymerase polymorphic populations preparations primers probes problems proteins Protocol quences reaction regions result sample slides species specimens stain was modified starch stock solution structure studies subunit taxa techniques temperature template tion tissue tree tube variation