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Purification of receptors
Molecular characterization of receptors
20 other sections not shown
3-adrenergic receptor acid affinity chromatography affinity column affinity gel aliquots alprenolol antibodies assay binding activity Biochem Biol bovine buffer cDNA cells centrifugation Chapter Chem cholate cleavage clones containing denatured described in Protocol detergent dialysis digitonin diluted domains dopamine receptors EDTA EGF receptor eluate elution endoproteinase enzyme equilibrated ethanol extracts filter filtration fractions G-proteins GABAA receptor gel-filtration gene gradient group 3 receptors homogenate HPLC hydrophobic Incubate interactions kinase labelling lectin ligand ligand-binding mAChRs membrane preparation membrane proteins method mg/ml molecular weight NaCl non-specific binding opioid receptors pellet peptides phosphate photoaffinity labelling plasmid PMSF polypeptide procedure proteases purification purified receptors radioiodination radiolabelled radioligand reagent receptor preparation receptor protein remove residues resuspend room temperature sample SDS-PAGE Section Sephadex Sephadex G50 Sepharose sodium solubilization solution specific stir structure subtypes subunits sucrose supernatant transfection transmembrane Tris-HCl Triton tube volume wash yohimbinic