In Situ Hybridization Techniques for the Brain
Wiley, Dec 11, 1996 - Science - 176 pages
Written by molecular biologists and neurobiologists, this volume in the Handbook Series provides basic step-by-step protocols in addition to specialist techniques to support those practising in situ hybridization, a powerful histochemical technique which is now widely used in neurobiology. The first part of the manual concentrates on basic procedures, eg, which probe and label to select, equipment, relevant molecular biological techniques, hazards, probe preparation and labelling, tissue preparation, and conditions for hybridization. Specialist procedures including quantitative analysis of data, reverse transcription PCR, working with human tissue, and double label methods are presented in subsequent sections. In Situ Hybridization Techniques for the Brain is, therefore, an essential reference source for all those with research interests in neuroanatomy, histopathology and/or neurohistochemistry, and will support users in both the preparation and successful completion of the procedures in their own laboratory.
18 pages matching hybridization mix in this book
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A Survey of the in Situ Hybridization Technique
Molecular Biological Techniques for in Situ Hybridization
In Situ Hybridization with 3SSLabelled Oligonucleotides 45
5 other sections not shown
acid agar agarose gels aliquots ammonium acetate analysis antisense autoclaved autoradiography background bacterial block brain cDNA cells cellular centrifuge Chapter Chesselet complementary concentration containing Coplin jars counterstaining cryostat cryostat sections DEPC DEPC-treated detection dH2O dipped DNA fragments emulsion enzyme ethanol ethidium bromide exposure film filter fixation formamide Fritsch and Maniatis frozen gene Histochem histochemistry hybridization buffer hybridization histochemistry hybridization mix hybridization signal Hybridization Techniques hydrolysis IBRO immunocytochemistry immunofluorescence incubation isotope labelled probe laboratory level of labeling messenger RNA method molecules mRNA neurons Neuroscience non-specific nucleotides oligonucleotide probes oligoprobes overnight pages paper paraffin paraformaldehyde plasmid plasmid DNA plasmid vector posthybridization washes preparation pretreatment primers probe solution procedure protease protein protocol quantification radioactive radiolabeled reaction reagents receptor RNA polymerase RNA probes RNase room temperature Sambrook sensitivity sequence silver grains slides sodium sterile synthesis target mRNA tissue section transcription volume