In Situ Hybridization Techniques for the Brain

Front Cover
Z. Henderson
Wiley, Dec 11, 1996 - Science - 176 pages
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Written by molecular biologists and neurobiologists, this volume in the Handbook Series provides basic step-by-step protocols in addition to specialist techniques to support those practising in situ hybridization, a powerful histochemical technique which is now widely used in neurobiology. The first part of the manual concentrates on basic procedures, eg, which probe and label to select, equipment, relevant molecular biological techniques, hazards, probe preparation and labelling, tissue preparation, and conditions for hybridization. Specialist procedures including quantitative analysis of data, reverse transcription PCR, working with human tissue, and double label methods are presented in subsequent sections. In Situ Hybridization Techniques for the Brain is, therefore, an essential reference source for all those with research interests in neuroanatomy, histopathology and/or neurohistochemistry, and will support users in both the preparation and successful completion of the procedures in their own laboratory.

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Contents

A Survey of the in Situ Hybridization Technique
1
Molecular Biological Techniques for in Situ Hybridization
31
In Situ Hybridization with 3SSLabelled Oligonucleotides 45
77
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