DNA Cloning: Expression systems
David M. Glover, B. D. Hames
IRL Press, 1995 - Medical - 274 pages
This book is the second of a revised set of four Practical Approach volumes on DNA Cloning which cover the major experimental techniques in use in modern molecular biology laboratories. The second volume, Expression Systems, is written by established researchers in the field. It provides essential background information and full details of current laboratory procedures for the expression of cDNA clones in E. coli, lambda-phage, yeast, and baculovirus expression systems, together with methods for screening libraries, isolation of proteins on the basis of protein-protein interactions, purification of the desired protein(s), and the generation of monoclonal and polyclonal antibodies. Each chapter contains key literature citations to acquaint and inform the reader, together with background information, up-to-date protocols, advice, hints, tips, and troubleshooting comments.
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