Human Insulin by Tryptic Transpeptidations of Porcine Insulin and Biosynthetic Precursors |
Contents
Introduction and background | 1 |
Alternative proteases | 21 |
pH measurements in mixtures of water and organic | 29 |
Copyright | |
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Other editions - View all
Human Insulin by Tryptic Transpeptidations of Porcine Insulin and ... J. Markusse No preview available - 2014 |
Common terms and phrases
absence of Ca2+ acetamide acetic acid acetic acid/acetate Acetone precipitation HPLC activity acyl-enzyme alanine amino acid autolysis Calcium Substrate Enzyme carboxylic acid chymotrypsin cleavage concentration of water Conditions Organic solvent coupling reactions decrease difference to 100 DMAC DMSO DMSO/BD DOI plus DOI-Thr-OMe Enzyme Analysis Sampling equilibrium constants ethanol Figure guanidinium hydrochloride H₂O HI-OBu HI-OR HOAc/OAc M/M human insulin esters hydrolysis increase insulin compounds insulin to human isocratic elution Table kinetic Ksyn lyticus protease Method Acetone precipitation molecule Morihara nominal Buffer M/M Organic solvent DMAC Organic solvent H₂O peptide bond plasmin porcine insulin porcine insulin FC porcine proinsulin porcine trypsin TC Preparation Method Acetone proinsulin proteins rate constants ratio reaction progress reaction rates residue Sampling h SCI-AAK simulation specificity q Substrate Enzyme Analysis Thr-OBu Thr-OMe Thr-OR Threonine ester Temperature transpeptidation reactions trypsin inactivation water concentration Yields of HI-OMe α α1 α β α βι α