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24 hours proestrus 72 hours postestrus Anat animal observed anticipated estrus appearance Ascheim atresia Atretic Follicles become beginning Commercial formalin Congestion copulatory response corpora lutea corpus iuteum Corpus luteum cumulus oophorus cyclic cytology determine the onset discus proligerus Doisy droplets embryonic embryonic-appearing cells estrogenic hormone estrus cycle fixed in Zenker's follicular antrum follicular cavities formation Geomys bursarius glandular granular cytoplasm granules guinea pig ovary Immature increase interstitial tissue Kansas Levi's solution mammals Mature follicles Mature Vesicular Follicles mitotic figures Mossman multiplication Non-vesicular onset of estrus Order osmic acid osmic stain ovulation ovum Papanicolaou 1917 period between 48 pocket gopher polyhedral cells predictions probably due Prom pyknotic nuclei Recently Ruptured sacrificed showing at least stain with osmic Stockard structure theca interna thecal cells thecal gland thesis tiie transform into interstitial typical University of Wisconsin unruptured follicle vaginal membrane vaginal smear vessels Westman Young