Sugar-lipid Intermediates Related to Colanic Acid and Lipopolysaccharide Produced in Vitro by Mucoid (lon, Or CapR) Mutants of Escherichia Coli |
Common terms and phrases
acceptor activity added addition amount appears assay bacitracin bacterial Biochem Biol biosynthesis bond calculated capsular carrier lipid cell wall centrifuged characterized Chem chromatography colanic acid column complete concentrations contained culture cytoplasmic described determined EDTA effect ELUTION enzymes Equation Escherichia coli extracted Figure final formation fucose galactose GDPF glucan glucose glucose-lipid glucuronic acid growth hydrolysis incorporation incubation indicate inhibition inhibitors involved isolated known labelled lines lipid intermediate lipopolysaccharide material mechanism membrane Methods minutes mixture mucoid lipid mucoid polymer mucoid system mutants normal nucleotide O-antigen oligomer P-lipid particulate Peak peptidoglycan percent phosphate phospholipid points polymer polymeric polysaccharide precipitate preparation present production protein purified pyrophosphate ratio reaction repeat unit requirement result Salmonella salt seen separated shown shows similar specific strains structure studied substrate sugar sugar-lipid suggested synthesis Table temperature thesis UDPG values vitro washed weight