DNA Fingerprinting in Plants: Principles, Methods, and Applications, Second Edition (Google eBook)

Front Cover
CRC Press, Feb 28, 2005 - Science - 472 pages
3 Reviews
Given the explosive development of new molecular marker techniques over the last decade, newcomers and experts alike in the field of DNA fingerprinting will find an easy-to-follow guide to the multitude of techniques available in DNA Fingerprinting in Plants: Principles, Methods, and Applications, Second Edition. Along with step-by-step annotated protocols, the authors fully discuss the technical aspects and modifications of existing techniques, the influence of reaction components and conditions, and the analysis of the results.

This second edition has been completely revised to address the exponential changes in the field since the first edition, focusing on PCR-based techniques but also including more sophisticated ones. The authors include numerous case studies to illustrate applications of the methods, more than 1600 references to the literature, and descriptions of reagent formulation, equipment, and computer programs used for evaluating molecular marker data. They compare the various methods, including the costs and benefits of each, helping readers determine which is best suited to a particular application.

The well-rounded, cross-referenced, and unified nature of this book makes it intrinsically easier to follow than the edited, multi-authored books currently available. It is an absolute necessity on the lab bench of anyone involved in plant research, DNA profiling, and molecular markers.

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An Important Source of Variation in Eukaryotic Genomes
Chapter 2 Detecting DNA Variation by Molecular Markers
Chapter 3 Laboratory Equipment
Chapter 4 Methodology
Chapter 5 Evaluation of Molecular Marker Data
Chapter 6 Applications of DNA Fingerprinting in Plant Sciences
Chapter 7 Linkage Analysis and Genetic Maps
A Comparison
Appendix 1 Plant DNA Isolation Protocols
Appendix 2 Commercial Companies
Appendix 3 Computer Programs Dealing with the Evaluation of DNA Sequence Variation and Molecular Marker Data
Appendix 4 Web Pages of Interest
Back cover

SNiPs and Chips for DNA and RNA Profiling

Common terms and phrases

Popular passages

Page 402 - Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase.
Page 362 - Feinberg AP and Vogelstein B. (1983). A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity.
Page 375 - G. VOSMAN B, MATTHES M. DALY A. BRETTSCHNEIDER R, BETTINI P. BUIATTI M. MAESTRI E, MALCEVSCHI A, MARMIROLI N. AERT R, VOLCK.AERT G. RUEDA J, LINACERO R, VAZQUEZ A, KARP A (1997), 'Reproducibility testing of RAPD, AFLP and SSR markers in plants by a network of European laboratories'.
Page 417 - D. (1987) A sequence in M13 phage detects hypervariable minisatellites in human and animal DNA. Science 235, 683-684.
Page 415 - Triglia, T., Peterson, MG, and Kemp, DJ (1988) A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences. Nucleic Acids Res. 16, 8186.
Page 375 - Jeffreys, AJ, Wilson, V., Neumann, R., and Keyte, J., (1988) Amplification of human minisatellites by the polymerase chain reaction: towards DNA fingerprinting of single cells, Nucleic Acids Res. 16: 10053-10971.
Page 414 - TC 1994. Comparison of RFLP and RAPD markers to estimating genetic relationships within and among cruciferous species. Theoretical and Applied Genetics, 88: 973-980.

References to this book

About the author (2005)

University of Frankfurt, Germany.

Swedish University of Agricultural Science.

University of Leiden, The Netherlands.

Khalid Meksem is Assistant Professor at the Department of Plant, Soil & General Agriculture of Southern Illinois University. After gaining his doctorate at the University of Cologne, Germany, he joined Southern Illinois University at the end of 1996. Dr. Meksem has taught courses on genomics and bioinformatics, as well as molecular genetics. His main research interests focus on
--Genomics tools for soybean: TILLING, developing high throughput tool for gene functional analysis and reverse genetics.
--BAC and physical mapping: physical map construction and integration
--Soybean Cyst Nematode disease resistance genes: Genetic diversity, additional genes for resistance and resistance pathways
--Plant pathogen genomics: Fusarium virguliforme structural and functional genomics, pathogenicity genes identification
--Developing international and national scientific network on structural genomics in plants (Comparative physical mapping) and functional genomics (TILLING)
Alongside registering four patents so far, Khalid Meksem is the associate editor of the Journal of Biomedicine and Biotechnology and a reviewer for a number of International Scientific Journals. He chaired the Functional genomic workshop at the International Plant & Animal Genome Conference in San Diego, and works as a reviewer for several granting agencies, including the National Science Foundation and the US Department of Agriculture.
Gunter Kahl is Professor for Plant Molecular Biology at Johann Wolfgang Goethe University, Frankfurt am Main, Germany. After gaining his Ph.D. in plant biochemistry, he spent two postdoctoral years at Michigan State University, East Lansing, USA, joining Professor JoeVarner and Professor James Bonner at the California Institute of Technology, Pasadena. His main research interests focus on
--Gene technology, in particular the genetic and physical mapping as well as the isolation and characterization of plant defence genes and their promoters, and the use of in vitro modified defence genes for the improvement of plant crops via gene transfer.
Plant genome analysis, in particular the development of molecular marker technologies and informative DNA markers for genomic fingerprinting, the establishment of genetic maps, the use of BAC libraries and physical mapping, and the map-based cloning of agronomically important genes.
--Expression profiling of plant tissues with expression microarrays and high-throughput techniques such as SAGE.
Due to the international nature of this work, Professor Kahl cooperates with a series of research institutions throughout Europe, in Japan, the USA, Syria, India, and South America. In addition, he is currently organizing a series of molecular marker courses in Austria, South America, Asia, Africa and the Middle East. He has also served in expert missions for IAEA, FAO and UNESCO in these and many other countries.
Professor Kahl is the author of more than 250 scientific publications, and is currently CSO at GenXPro, a company specializing in novel technologies in genomics and transcriptomics, located at the Frankfurt Innovation Center for Biotechnology (FIZ), Frankfurt am Main, Germany.

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