Detection and Isolation of Soil FungiAlthough there has been remarkable progress in the detection and identification of micromycetes in recent years, we still resort to empirical methods to obtain a pure culture of a fungus from a soil sample or from an infected root. Some of these methods have been reproduced in this book, just as they were first published in journals. It is for the first time that these methods have been collected in one volume. Regardless, how empirical these methods may be, they are based on some base data which is described in the first part of the book. It can be used to develop other techniques, or for preparing new selective media. The techniques and isolation media for a given fungus are presented in the second part. The species are listed in alphabetical order. Indices are given for easy reference. This concise book is intended for all those who handle soil fungi, whether for theoretical studies or practical applications. |
Contents
Isolation Techniques | 6 |
Isolation from Roots | 14 |
Principal Selectivity Factors | 22 |
Copyright | |
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Adjust the pH agar distilled water agar medium analysed antibiotics Aphanomyces autoclaved and warm autoclaving baiting Beauveria bassiana benomyl Bouhot centrifuge chloramphenicol chlortetracycline colonies containing cornmeal agar culture detection discs disinfect filter paper flasks fungicides fungus g of agar g of soil g/l plain agar glucose growth homogenise hymexazol incubation at 25°C inhibited inoculum Isolation from infected Isolation from soil isolation medium litre malt extract metalaxyl mg 50 mg mg Isolation mg/l microsclerotia ml After autoclaving ml of sterile molten nystatin observed oxgall Papavizas parasitica pathogen PDA medium penicillin Petri dishes Phytopathology Phytophthora cinnamomi pimaricin plants prepare a suspension Pythium quintozene Rhizoctonia solani rifampicin rose bengal sclerotia seedlings selective medium sieves with meshes sodium hypochlorite soil sample soluble species Spread 1 ml sterile distilled water sterile water sterilised streptomycin sulphate substrate sucrose supernatant surface technique temperature vancomycin Verticillium warm medium wash zoospores µm mesh