PCRThis up-to-date text provides both an introduction to PCR (polymerase chain reaction) for those new to the technique, and coverage of the wide range of current applications. PCR has revolutionized and underpins modern molcular biology. By focusing on current PCR technology, the book should be of interest to both first-time users and experienced researchers, as well as for the bioscience undergraduate. |
Contents
Instrumentation Reagents and Consumables | 9 |
Amplifying the Correct Product | 27 |
12 | 36 |
Copyright | |
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Common terms and phrases
Acids Res allele amplicon Amplitaq analysis annealing applications ARMS primer assay asymmetric PCR base pairs cell chemical chromosome cloning complementary concentration contamination cycle deletion denaturation detection DGGE DNA fragments DNA molecule DNA sequence dNTPs dot blot double-stranded exon exonuclease exonuclease activity extension fluorescent gel electrophoresis genetic genomic DNA GSP1 heteroduplexes human hybridization incorporated labeled Laboratories lane ligation melting microsatellites mismatches mRNA multiplex mutagenesis mutagenic mutations Natl Acad normal Nucl nucleotide oligonucleotide parallel DGGE PCR amplification PCR Methods PCR primers PCR products PCR reaction performed Perkin-Elmer Cetus Perpendicular DGGE plasmid polymorphisms primer design primers probes Proc protein radiolabeled RAPD reagents recombinant region residues restriction enzyme restriction enzyme digestion restriction enzyme recognition reverse transcriptase sample shown in Figure single Southern blot SSCP synthesis Taq DNA polymerase target DNA target gene technique temperature thermal cycler thermostable DNA polymerases transcription UK distributor vector vectorette unit