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Definition and Significance
Harvest Purification and Viability
15 other sections not shown
Agrobacterium Agrobacterium tumefaciens amino acid Appl auxin auxotrophs bacterial Biol Brassica callus Callus formation carrot cell cultures cell fusion cell hybrids cell lines cell suspension cell wall cellulase chloroplast chromosomes clones crop cryopreservation cryoprotectant culture of protoplasts cytoplasmic Datura innoxia Daucus density enzyme freezing frequency fused Genet genome glauca growth haploid higher plant increased induced inhibition isolated protoplasts isolation of protoplasts leaf protoplasts Lett liposomes male sterile Maliga medium mesophyll protoplasts metabolic method mitochondria mutants Nature London Nicotiana tabacum nitrate nopaline nuclear octopine osmotic osmoticum Petunia Pflanzenphysiol Plant Cell Rep plant cells Plant Physiol plant protoplasts Plant Sci plants regenerated plasmid possible proline proteins protoplast culture protoplast fusion protoplasts isolated recombination reductase selection Solanum somaclonal somatic hybrids species storage streptomycin suspension cultures sylvestris synthesis T-DNA temperature Theor Ti-plasmid tissue culture tobacco cells tobacco protoplasts transfer tumefaciens tumor uptake variants vector virus vitro