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0.5 per cent active immunity active virus agent animals developed antibodies applied bled for test cent picric acid cent sine cent virus suspension central nervous system chemical treatment convalescent monkeys day Polio days after treatment developed the disease doses of virus epidemic experiment are given experimental five successive days Flexner and Lewis formaldehyde formalin treated virus four given in Table immune horse serum immune serum immunisation immunized monkeys inactivated injected intracerebrally inoculated intranasally inoculated with virus intra intranasal inoculation intranasal route Levaditi lithium carbonate mercurochrome MID of virus monkeys treated month after treatment myelitis nerve cells neutralised Oebhardt olfactory mucosa olfactory tracts paralysis period piorio Polio Oth day poliomyelitis poliomyelitis virus prophylactic prophylactic value protective action Schults sodium chloride solution sprayed subcutaneous tested for resistance three monkeys tissue treated animals treated monkeys Twelve monkeys twenty-four hours vaccine virua virus inoculations zinc sulfate