Enzymes of secondary metabolism
Peter J. Lea
Academic Press, 1993 - Science - 478 pages
The series, Methods in Plant Biochemistry, provides an authoritative reference on current techniques in the various fields of plant biochemical research. Each volume in the series will, under the expert guidance of a guest editor, deal with a particular group of plant compounds. Each will describe the historical background and current, most useful methods of analysis. The volumes include detailed discussions of the protocols and suitability of each technique. Case treatments, diagrams, chemical structures, reference data, and properties will be featured along with a full list of references to the specialist literature. Conceived as a practical companion to The Biochemistry of Plants, edited by P.K. Stumpf and E.E. Conn, no plant biochemical laboratory can afford to be without this comprehensive and up-to-date reference source.
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Enzyme assay techniques
Conclusions and perspectives
18 other sections not shown
2-mercaptoethanol Academic Press acetate acetone alkaloids amino acid ammonium sulphate Arch ascorbate assay Biochem Biochemistry Biol Biophys biosynthesis carotenoids catalyses centrifuged Chem chemical chlorophyll chloroplasts chromatography cofactor column compounds concentration Conn containing Croteau crude extract cyanogenic glycosides cyanohydrin cysteine synthase cytochrome P-450 EDTA eluted enzymatic enzyme activity enzyme preparation ester etal flavonoid Forkmann formation fractions gel filtration glucose glucosinolate Grisebach Halkier higher plants homogeneity homogenised HPLC hydroxylase hydroxylation Ikegami incubation inhibition inhibitors intermediates Isoenzyme isolated lyase M0ller membrane metabolism method methyl microsomal molecular weight molecule monoterpene Murakoshi myrosinase NaCl NADPH Naturforsch oxidase oxidation partially purified pathway phenolic Phytochemistry phytoene Plant Physiol plastid Poulton precipitate precursors prenyltransferases procedure protein purification pyrophosphate radioactivity reaction mixture reagent reductase saturation seedlings Sephadex sodium specific activity step Strack substrate supernatant synthesis tissue total volume Tris-HCl buffer Underhill urogen yield Zenk