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acetate acetone action of thrombin active substance activity coefficient amount of thrombin amount of thrombokinase anticoagulant antithrombin Astrup & Darling autocatalytic autocatalytic reaction Biochem Biol blood clotting blood coagulation blood plasma Bordet plasma brain thrombokinase buffer of constant buffer solutions calcium chloride carried cent Chem chicken plasma citrate clotting process considerably constant ionic strength constant molarity curves obtained determination diluted enzymatic reactions enzyme equation experiments fibrin fibrinogen solution Fischer Glazko heparin hydrochloric acid Ibidem inactivation increase influence inhibition inhibitory substances investigations isoelectric point kinase large amounts measurements Mellanby method mixture neutral salts ox brain ox lung oxalated ox plasma pH optimum pH value phosphate buffer Physiol possible potency precipitation preparation present properties protein prothrombin reaction optimum reaction velocity salt concentration shown sodium chloride stances studies substrate throm thrombin action thrombin formation thrombin on ox thrombin solution Thrombinets thrombo thromboplastin tissue factor varying Virkning Volkert