Vanilmandelic Acid & Catecholamine Determinations |
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Page 47
... VMA band is eluted with alkaline methanol and quantitated spectrophotometrically at 520 nm . Reagents 1. Sodium nitrate , 0.1 % ( wt / vol ) Refrigerate . 2 . Alkaline methanol solution 2 % ( wt / vol ) sodium carbonate 100 ml ...
... VMA band is eluted with alkaline methanol and quantitated spectrophotometrically at 520 nm . Reagents 1. Sodium nitrate , 0.1 % ( wt / vol ) Refrigerate . 2 . Alkaline methanol solution 2 % ( wt / vol ) sodium carbonate 100 ml ...
Page 50
... is of a urine sample obtained from a normal patient after ingestion of aspirin . Electrophoresis was on Gelman Sepraphore III cel- lulose acetate strips at 2 mA for two hours . 4 . Our VMA band is located 6-6.5 cm from 50.
... is of a urine sample obtained from a normal patient after ingestion of aspirin . Electrophoresis was on Gelman Sepraphore III cel- lulose acetate strips at 2 mA for two hours . 4 . Our VMA band is located 6-6.5 cm from 50.
Page 60
... VMA band , as well as the other phenolic acids , can be quantitated by densitometry after staining compared with standards run on the same plate . Working time is about 30 minutes . Reagents 1. Sodium nitrite , 0.5 % ( wt / vol ) 2 ...
... VMA band , as well as the other phenolic acids , can be quantitated by densitometry after staining compared with standards run on the same plate . Working time is about 30 minutes . Reagents 1. Sodium nitrite , 0.5 % ( wt / vol ) 2 ...
Contents
Introduction | 3 |
Formation of Vanilmandelic Acid | 11 |
CatecholamineSecreting Tumors | 19 |
Copyright | |
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Common terms and phrases
acid reagent acid VMA adrenal medulla aliquot alkaline ascorbate beaker Bio-Rad BIOMED blood pressure Catechol catecholamines cellulose acetate cellulose acetate strips centrifuge tubes chromaffin clinical color column COMT concentrated hydrochloric acid creatinine cuvets demineralized water diastolic diazotized diazotized p-nitroaniline dilute distilled water DOPamine electrophoretic elevated eluate enzyme epinephrine epinephrine and norepinephrine ethyl acetate excretion of VMA Figure filter paper hormones hydrochloric acid hypertension Increased Increased metabolism metanephrine method MHPG minutes ml of alkaline ml of Solution ml urine monoamine oxidase Neuroblastoma norepinephrine normal normetanephrine OH OH oxidation patients with pheochromocytomas phenolic acids pheochromocytoma pipette potassium carbonate procedure purple-blue reaction reagent grade recovery refrigerator sample blanks sodium nitrite specimens stain sulfate sympathetic nerve endings systolic test tube Toluene tyramine Tyrosine unknown urinary excretion urinary VMA urine urine sample urine volume Vanillin vanilmandelic acid VMA band VMA standard volumetric flask wt/vol ОН