Botanical Microtechnique |
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Page 61
... hemalum . Many modi- fications may be found in the literature . The term " hemalum " is used in this manual to refer to any of the self - mordanting alum hema- toxylins . The choice among these stains is a matter of personal preference ...
... hemalum . Many modi- fications may be found in the literature . The term " hemalum " is used in this manual to refer to any of the self - mordanting alum hema- toxylins . The choice among these stains is a matter of personal preference ...
Page 66
... HEMALUM ( REGRESSIVE ) The destaining action of acids on hematoxylin is selective , — cytoplasm is destained more rapidly than cell walls , plastids , and nuclear structures . This fact makes it possible to use a self - mordanting ...
... HEMALUM ( REGRESSIVE ) The destaining action of acids on hematoxylin is selective , — cytoplasm is destained more rapidly than cell walls , plastids , and nuclear structures . This fact makes it possible to use a self - mordanting ...
Page 67
... Hemalum With " General " Counterstain Pre - Staining Operations and Intervals as in Chart I Hemalum to correct intensity ↓ 30 % alcohol ↓ 50 % alcohol ↓ 70 % alcohol ↓ 95 % alcohol ↓ erythrosin ( see text ) resin and cover glass ...
... Hemalum With " General " Counterstain Pre - Staining Operations and Intervals as in Chart I Hemalum to correct intensity ↓ 30 % alcohol ↓ 50 % alcohol ↓ 70 % alcohol ↓ 95 % alcohol ↓ erythrosin ( see text ) resin and cover glass ...
Contents
Introduction | 3 |
Collecting and Subdividing Plant Materials | 5 |
Killing Fixing and Storing Plant Tissues | 17 |
Copyright | |
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absolute alcohol acetic acid acetone alcohol-xylene algae anhydrous anther aqueous binocular bottle brittle buds butyl alcohol camera cell walls celloidin chloroform chrome-acetic chromic acid chromosomes color condenser counterstain cover glass Craf cytological dehydration destaining dioxan disk dissect embedding embryo erythrosin ethyl alcohol excellent fast green filamentous fixation focus formaldehyde formula gametophytes glycerin hardened hemalum hematoxylin infiltration intervals iron hematoxylin killed in FAA killing fluid knife leaf leaves lens lignified longitudinal magnification material melted methods microscope microtome mounting block objective obtained ocular orange G organs oven paraffin parfocal permanent slides photomicrographs photomicrography pieces plants plasmolysis preparations preserved reagent removed resin ribbon rinse root tips safranin safranin-fast green satisfactory sections smear solvent species specimen sporocytes sporophylls stage Stain Tech Staining Chart stem structure subjects thick tissues transverse tube twig wash whole mounts wood woody workers xylene