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Materials and Methods
Results and Discussion
7 other sections not shown
0FTR amino acid amplified analysis Anderson and Welsh aspartic acid assay associated ATP binding ATPases base pair CF chromosomes CF gene CF mutations CF patients CFPAC-1 cells CFTR function CFTR variants channel activation chemical mismatch cleavage chromosomes cleavage product clones consensus sequence constructs cystic fibrosis digested dose-response relationships Drumm epithelial exon 12 Figure forskolin forskolin and IBMX fragments frameshift G551S mutation glycine glycine 551 homozygous hybridized IBMX IBMX dose-response relationships identified incubated indicated intron intron 12 Lanes lysine mild disease minutes missense mutations mRNA mutagenesis mutation detection N/A N/A N/A NBF mutations NBF1 and NBF2 NBF1 Walker nucleotide nucleotide binding folds nucleotide binding pocket nucleotide sequence oligonucleotide oocytes oocytes expressing pancreatic insufficiency phosphorylation piperidine primers residue resuspended screening sensitivity of CFTR serine splice donor subcloned substitution sweat traffic ATPases transfected Walker A motif wild-type wild-type CFTR Xenopus oocytes Xhol