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9-octadecenoic acid methyl esters albumin amounts analysis Asselineau and Lederer atmosphere at 20 Bacteriol Biochem Biol biotin boiled serum medium carbon atoms Cell Mass cells grown cent dry weight cent rabbit serum Chem chemically characterized medium complex lipids components cultivation of leptospirae cyclopropane cyclopropane acids days with static determine dried esters ethanol ethanol-ether extracts extracted with ethanol-ether fatty acid methyl fraction free fatty acids gas chromatograph glycerides gram-negative growth factor hydroxybutyric acid icterohaemorrhagiae Infrared spectra infrared spectroscopy J. B. Wilson Lactobacillic acid Lactobacillus Lactobacillus acidophilus leptospirae leptospiral cells leptospiral lipids lipid composition lipid content lipid material lipids extracted lipids were subjected metabolism methyl Mycoplasma gallisepticum oleic acid organisms palmitic acid pathogenic leptospirae plates pomona pornona rabbit serum-buffer medium reported retention saturated serotypes serum protein solvent Stalheim standard fatty acid stimulated strains studies synthesis synthetic medium thesis Thin layer chromatography total lipid University of Wisconsin unsaturated fatty acids