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anthracene approximately aqueous phase bacteria biodegradation of DEHP biosurfactants cell densities cells per ml chemical concentration of DEHP concentration of phthalate concentration of solubilizer conditioned media crude solubilizer culture fluid Cytophaga sp DEHP by Cytophaga DEHP by Mycobacterium DEHP by Nocardia DEHP per ml DEHP remaining determinations and error DIOP disappearance of DEHP distilled deionized water EDTA enrichment medium enzymes equilibrium concentration error bars represent esters experiment growth media growth of Cytophaga hexadecane Hours Fig hydrocarbon inoculated inorganic salts solution insoluble isolates lag phase lipophilic mean of triplicate mechanisms of uptake Microbiol microorganisms Mineralization of DEHP Minitab Mycobacterium sp Mycobacterium sp.-conditioned media Mycobacterium-conditioned media naphthalene Nocardia sp organisms phenanthrene phenethylamine phthalate phthalate esters potential present study Primary biodegradation produce solubilizers protease represent one standard solubilization assay solubilizers produced source of carbon sparingly soluble substrates specific standard deviation triplicate determinations tubes unconditioned untreated conditioned media uptake of DEHP vial