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absorption acetone activity adsorbent aliquot alkali amount Anal anhydrous animal antimony trichloride ascorbic acid autoclave beaker Biochem Biol biological biotin blank Blendor buffer Calculation carotene casei centrifuge cereals Chem chemical methods chick chloroform choline CNBr color colorimeter colorimeter tube concd concentration containing cryptoxanthin Decalso dehydroascorbic acid density determination of vitamin diketogulonic acid Dissolve enzyme Erlenmeyer Flasks factor filter filtrate fluorescence folic acid foods fresh galvanometer reading growth HPO3 incubation inoculum isobutyl alcohol laboratory Lactobacillus liter liver microbiological assay microbiological method minutes ml of water ml with water NaOH niacin nicotinic acid Nutrition obtained oxidation pantothenic acid petroleum ether pigments Pipets Preparation procedure pyridoxine quantities reaction Reagent riboflavin sample saponification soluble solution Reagent solvent standard curve stock solution temperature test tubes thiamine thiochrome tion tissues titration tocopherols tubes add values vitamin B6 volume volumetric flask volumetric transfer wash water bath Weigh yeast