Electron Microscopic Immunocytochemistry: Principles and Practice
Julia M. Polak, John V. Priestley
Oxford University Press, 1992 - Medical - 267 pages
Electron microscopic immunocytochemistry is a powerful technique combining the resolution of the electron microscope with the specificity and sensitivity of immunocytochemistry. This book discusses methods designed to make this intricate procedure more accessible, and covers recent developments that have simplified the technique and broadened the range of applications. It presents the principles of EM immunocytochemistry, detailed protocols, and examples of ways in which the technique can be applied to a variety of fields, including biomedical research and diagnostic pathology. The book begins with an overview of EM immunocytochemical procedures and of applications in histopathology. The three major chapters cover post-embedding, pre-embedding, and ultra-thin cryosection techniques. The final section illustrates applications of EM immunocytochemistry to the study of endocrine tumors, renal disease, neuropathology, dermatology, and microbiology. The emphasis throughout this detailed volume is on providing practical guidance to scientists and clinicians who may be using the technique for the first time.
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Electron microscopic immunocytochemistry
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acid adenomas Anat antisera antiserum applications Bendayan block bullous pemphigoid calcitonin carcinoid carcinomas Chapter collagen colloidal gold cryosections Cuello Cytochem cytochemistry cytoplasm dehydration deposits diagnostic disease electron microscopic electron microscopic immunocytochemistry embedding endocrine cells endocrine tumours enzymes epithelial ferritin filaments fixation fixative gastrinomas glomerular basement membrane glutaraldehyde glycoproteins gold labelling gold particles gold probes grids Histochem Histochemistry hormone human immuno immunocytochemical localization immunocytochemistry immunoelectron immunoelectron microscopic immunoglobulins immunogold labelling Immunolabelling for electron immunoperoxidase immunoreactivity immunostaining incubation J. M. Polak keratin lamina light and electron light microscopic markers methods Microsc microscopic study monoclonal antibodies morphological neuroendocrine tumours neurofibrillary tangles neurons nm gold normal osmium osmium tetroxide pancreatic paraformaldehyde Pathol penetration peptides peroxidase Pickel pituitary post-embedding pre-embedding staining Priestley primary antibody protein protein A-gold reagents renal resin Scale bar secretory granules specific surface antigens syndrome technique temperature tion tissue Ultrastructural localization ultrathin sections Warhol