Electron Microscopic Immunocytochemistry: Principles and Practice

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Julia M. Polak, John V. Priestley
Oxford University Press, 1992 - Medical - 267 pages
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Electron microscopic immunocytochemistry is a powerful technique combining the resolution of the electron microscope with the specificity and sensitivity of immunocytochemistry. This book discusses methods designed to make this intricate procedure more accessible, and covers recent developments that have simplified the technique and broadened the range of applications. It presents the principles of EM immunocytochemistry, detailed protocols, and examples of ways in which the technique can be applied to a variety of fields, including biomedical research and diagnostic pathology. The book begins with an overview of EM immunocytochemical procedures and of applications in histopathology. The three major chapters cover post-embedding, pre-embedding, and ultra-thin cryosection techniques. The final section illustrates applications of EM immunocytochemistry to the study of endocrine tumors, renal disease, neuropathology, dermatology, and microbiology. The emphasis throughout this detailed volume is on providing practical guidance to scientists and clinicians who may be using the technique for the first time.

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