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1.0 mg carbonyl-^C-desthiobiotin Achromobacter liquifaciens grown addition of pimelic ammonium arabinosus Aspergillus niger grown avidin azelaic acid Bacillus bacteria biosynthesis of biotin biosynthetic pathway biotin activity biotin biosynthesis biotin from desthiobiotin biotin production biotin requirement biotin sulfone biotin synthesis biotin-1-sulfoxide C-biotin sulfone isolated carbon carbonyl carrier biotin casei cerevisiae CH^CH^COOH chromatography chrysogenum grown coli column compounds conversion of desthiobiotin convert desthiobiotin converted to biotin Corynebacterium diphtheriae culture filtrate cystine days growth desthio desthiobiotin to biotin determined Eakin Eisenberg eluent filtrate of Aspergillus grown with 1.0 isolated from Achromobacter isovaleric acid Lezius lipoic acid medium methionine microorganisms ml of media Norit paper chromatography pathway of biotin pelargonic acid Penicillium chrysogenum peroxidized pimelic acid radioactive radioactive biotin radioactive desthiobiotin Raney nickel Recrystallization Portion Radioactivity recrystallizations of C-biotin require biotin resting cells sitophila Sordaria fimicola sphaericus strain Successive recrystallizations sulfur synthesis of biotin synthesis of desthiobiotin tested unknown vitamer yeast