Genome Analysis: Analyzing DNACold Spring Harbor Laboratory Press, 1997 - Gene mapping |
Contents
Purifying and Analyzing Genomic DNA | 1 |
DNA FROM YEAST | 26 |
DNA FROM BACTERIA | 34 |
Copyright | |
24 other sections not shown
Common terms and phrases
agarose gel agarose plugs aliquots Amount to add amplification Appendix for Caution approximately bacterial bacteriophage beads buffer cDNA cells centrifuge chromosome Component and final containing contigs culture database denaturation DNA concentration DNA fragments DNA molecules DNA polymerase DNA sample DNA sequencing dNTP EcoRI EDTA EDTA pH 8.0 electrophoresis equilibrated ethanol ethidium bromide exonuclease final concentration final volume gene genomic genomic DNA gradient H₂O HMW DNA hybridization Incubate at 37°C insert lane ligation lysis mammalian mapping medium membrane method methylase mg/ml microcentrifuge tube minutes Note nucleotide oligonucleotide partial digestion PCR products pellet PFGE phenol pipette tip plasmid plate precipitation primer probe protein proteinase K protocol purified quencing RARE cleavage restriction enzyme Resuspend room temperature shotgun sequencing single-stranded solution spheroplasting step stored at 20°C subclones supernatant TBE buffer template DNA tion transfer transposon Tris-Cl vector vector DNA vortexing yeast µg/ml