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The Basic Theory of Chromatography and Some General
Chemical structure and chromatographic behaviour
30 other sections not shown
17-oxosteroids acetic acid acetic acid system acetylation Acta adsorption Ai?M aldosterone alkaline Amer ARMg ARmq ARms ARmt benzene Biochem biol bismuthate bismuthate oxidation Bush and Mahesh Bush and Willoughby Celite cellulose Chem chim chromato chromatogram chromic acid colour column compounds concentration conjugates cortisol derivatives diacetate dissolved eluate elution Endocrin epimeric esters estimation ether ethyl acetate evaporated extract fluorescence fluorimetry fraction give glucuronosides hydrocarbon hydrolysis hydroxide hydroxyl groups impurities interactions ketone ketone groups Lewbart material methanol methyl methylene chloride mixture mobile phase modifying step molecules NaOH Neher non-polar obtained oxidation paper chromatograms partition polar groups probably procedure progesterone propylene glycol quantitative reagent Reichstein Rm values room temperature scanning sensitivity separation silica gel sodium solution solvent systems specific stationary phase steric steroids strip substance substituents sulphate t-butanol Tait technique tetrahydrocortisol typical ultraviolet unpublished urine usually washed Zaffaroni Zimmermann reaction zone