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Practical Suggestions for Medium Selection and Handling
SCALINGUP OF ANIMAL CELL CULTURES
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acid agar ampoules antibodies antigens assay autoradiographic buffer Cancer cell culture cell density cell growth cell lines cell numbers cell population cell suspension cell type centrifugation chamber cloning clot collagen concentration containing coverslip culture vessel cytometry cytotoxicity detection diameter differentiation diluted distilled water drug elutriation embryos enzyme epithelium ethanol explants exposure fibroblasts Figure filter flask flow cytometry fluorescence glass growth factors growth medium HBSS Hormonally Defined Media hybridisation hybridoma ig/ml incubation laboratory laser MCDB method microcarrier mixing monolayer mycoplasma ng/ml obtained organ culture oxygen Pasteur pipettes perfusion Petri dishes pipette plasma plastic plate prepared procedure proliferation propidium iodide protein pump Reagents remove rinse rotor sample sensitivity serum slides solution specific spheroids stain sterile sterilisation substrate suitable surface area suspension culture technique temperature tissue culture transfer transferrin trypsin tubes viability vitro vivo volume wash watchglass