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14C-labeled 2-aminoadipic acid 3-nitropropionic acid 6-methylsalicylic acid A-type A-type colony acetate acetylCoA Acta actino actinomycin actinomycin synthesis activity addition amino acid ammonium sulphate antibiotic production Antibiotic Substances antibioticus aromatic ation Biochem biogenesis Biol Biophys biosynthesis carbohydrate carbon carboxyl cell cell-free cephalosporin chain Chem chemical chloramphenicol compounds concentrations configurational COOH cultures D-valine decarboxylation derived diploid disintegrations/min effect enzyme erythromycin fatty acid fermentation formation formed fungi Gatenbeck genetic glucose growth hydroxystreptomycin incorporation incubation inhibition intermediate intracellular involved isolated labelled lactone macrolide macrolide antibiotic malonate medium metabolism methyl Microbiol microorganisms molecule mutants mycelium mycin normal obtained OH OH OH oleandomycin organism orsellinic acid oxidation oxygen parasexual cycle pathway pattern penicillin peptide peptide antibiotics phenols phenoxazinone pigment polyketide possible precursor present Proc protein synthesis radioactivity reaction residue ring sarcosine Scheme secondary metabolites shown specific stipitatic stipitatic acid Streptomyces structure studies substrate sugars Table tetracycline valine yield