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24 hours absolute alcohol acid-fast bacilli amboceptor amount animals antiformin antigen avian bacteria blood boiling bottle bovine broth carbolfuchsin carbolic acid celloidin cells cent alcohol centrifugalized clinical color complement Complete hemolysis containing culture cutaneous decolorization diagnosis dilution disease distilled water dose drop filter fixation fluid glycerine grow guinea pigs heat hemolysis Complete inhibition human bacilli incubator infection inhibition of hemolysis inoculation intracutaneous laboratory large numbers latter lesion Loomis-Kolmer Technique lungs lymph nodes lymphatics medium method methylene blue microtome minutes needle non-pathogenic acid-fast normal occasionally paraffin pathogenic patient Petroff Technique physiological saline pipette placed preparation present procedure produce pulmonary rabbits react reaction saline solution sections sediment serum skin slide smear smegma sodium sodium hydroxide specimen sputum staining sterile subcutaneous surface suspension syringe temperature tion tissue titration tubercle bacilli tuberculin tuberculosis tubes urine usually virulence washed Wassermann Wassermann test weeks xylol