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alcohol ammonium acetate angles Araldite Araldite mixture bacteria benzoyl peroxide Biochem biological Biophys buffer carbon film catalyst cells centrifugation CHAPTER chemical chloroform colour cutting edge cutting thin sections Cytol dehydration dense desirable Diamond knives distilled water electron micrographs electron microscope Embedd1ng embedding medium emulsion epoxide ethanol evaporating exposure ferritin FIXATIVE Solution floating floating film formaldehyde Formvar gelatin capsule glass knives Glauert high contrast highest resolution light microscope liquid material membranes merisation methacrylate embedding methods microtome mins minutes monomer monomer mixture mounting negative staining Nucleic object osmium tetroxide OsO4 particles phospholipid phosphotungstic acid piece of tissue platinum polymer polymerisation damage Potassium permanganate preparation Prepolymerised problem procedure protein reaction refrigerator resins SCHEDULE scratches serial sections shadow slide small block sodium specimen stabilise structure supporting film technique temperature thick trimming uranium salts uranyl acetate usually vacuo Vestopal WASHING AND DEHYDRATION water bath water surface