Purification tools for monoclonal antibodies
Purification Tools for Monoclonal is an essential book for professionals, educators and advanced students in the field of Biotechnology. It is based on experience gained from purification process development, scale-up, and manufacture of more than 250 monoclonal-based diagnostic and therapeutic products. Ten chapters provide in-depth coverage of major separation mechanisms, process strengths, weaknesses and method development; all fully integrated with the special performance, economic and validation requirements associated with monoclonals. Covered methods include precipitation with inorganic salts, polyethylene glycol, electrolyte depletion, caprylic acid, ethacridine, chromatographic purification by size exclusion, ion exchange, hydroxyapatite, hydrophobic interaction, immobilized metal affinity, hydrophilic interaction, euglobulin adsorption, thiophilic adsorption, protein A, protein G, lectin affinity, and more. 88 figures, 29 tables.
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With polyethylene glycol
Size Exclusion Chromatography
11 other sections not shown
0.1M sodium chloride Academic Press Acta activity recovery adsorption affinity albumin ammonium sulfate Anal anion anti Bacterial BICINE binding buffer Biochem Biochemistry Biol BioPharm Biophys Boyle buffer exchange cation exchange cell charge Chem Chromatogr clearance column complexes conductivity contaminants denaturation dissociation dynamic capacity effects elution endotoxin equilibration ethacridine euglobulin evaluate exchange chromatography Figure flow rate foulants fractionation Gagnon glycol high salt HILIC histidyl hydrophobic interactions IMAC immobilized Immunol indicate ionic ions leachate leached protein lectin ligands limitations linear gradient loading lyotropic mass recovery matrix metal method development Molecular monoclonal antibodies Mouse octanoic acid on-line dilution peak Porath pore protein G protocol purification performance purity range reduced removal residues salt concentrations salt precipitation sample application scale-up Scand selectivity sodium chloride sodium phosphate specific step strongly supernatant tein temperature Timasheff tion titration variations virus vitro volume