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aberrant mutants acrasin acrif-R acriflavine hydrochloride aggregateless aggregateless mutants aggregation assayed bacterial associate bacterial lawn buffered water agar cells cellular slime molds chemical agents colonies compounds concentration cyc-R cyclic AMP cycloheximide developmental mutants developmentally aberrant Dictyostelium discoideum diploid doubly resistant doubly selective medium drug resistant efficiency of plating eth-R flasks fruiting structures G-S medium genetic growth medium haploid heterozygous heterozygous diploid incubation induce inhibited inhibitor inoculated inoculum L-canavanine sulfate liquid culture markers medium containing meiosis mixtures morphogenesis mutagen mutants isolated myxamoebae nitrosoguanidine non-selective medium normal fruits normal sorocarps observed parasexual cycle phosphate buffered water plaque formation plates of G-S produce normal Raper recombinants resistant clones resistant mutants segregants selective agents selective and non-selective sexual cycle Sinha and Ashworth sori sorocarp formation sorocarp production spores spread stalk strains Sussman and Sussman synergism synergistic response syngamy tested tion wild type Wilson